Biography
Abstract
Hepatitis C virus infection represents one of the major health problem that affects human population all over the world. In Egypt 10%-20% of the general population is infected with HCV. Type I interferon was secreted from the intrahepatic immune cells during the early days of infection followed by production of Interferon β (IFN-β) as indicated in HCV infected chimpanzee animal model. At the same time, HCV starts to attenuate the immune response and the production of specific HCV immune clearance. Blue green algae especially Spirulina species was well studied of its modulation on human immune response. In the present study, whole lysate from non-toxic blue green algae isolated from rice fields at River Nile delta in Egypt identified as Nostoc sp EGY (Accession number: JQ220407 and JQ220408) was used in order to determine the exact effect of this lysate on immune response during HCV infection. Our data showed that the component of this lysate can induce immune stimulation of human Peripheral Blood Mononuclear Cells (PBMC) at concentration range of 10-500μg/ml as well as viral clearance at low concentration as of 1 ng/ml. On the other hand, concentrations of this crude lysate ranging from 100μg-10mg/ml can stimulate normal human neutrophils to ingest (phagocytosis) and kill life Candida in an in vitro test. This indicates that total crude extract from non-toxic blue green algae can represent good candidate for preparation of safe and effective immune therapy for HCV treatment.
Biography
Abstract
Background & Aim: Migraine is common headache disorder and can lead to disability. Mostly analgesics are being prescribed having numerous side effects. Wet cupping is the part of regimental therapy as a treatment mode of migraine disease in Unani system of medicine. The aim of the study is to analyze the effectiveness of wet cupping (HIJAMA) among Pakistani patients suffering from migraine. Method: 250 patients were selected in a randomized 18 months’ clinical study from August 2013 to February 2015 at SADRI Health Services Islamabad, Pakistan. The age of the patients was 20 to 80 years, having no previous history of brain injury. 3 to 7 sessions of wet cupping were done on alternate days. The effectiveness of therapy was observed by using visual analogue score and PSQI sleep quality score. Data were analyzed by paired t test and ANOVA. Result: Out of 250 patients 60% (150) were female and 40% (100) were male. Average duration of migraine was 4.6 years. 58% (147) patients were having pain score more than 5. While after treatment there was significant decline in pain score i.e. 14.8% (37 patients). Moreover, the quality of sleep has also been improved from 56-13% as indicated by PSQI Score. Conclusion: After end of therapy it was concluded that wet cupping has shown improved efficacy in the management of migraine.