Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series : World's leading Event Organizer

Back

Bruno O Onyemegbulem

Bruno O Onyemegbulem

University of Jos, Nigeria

Title: Post-traumatic stress disorder and the toxicology of Cannabis sativa

Biography

Biography: Bruno O Onyemegbulem

Abstract

Many young men, women and even the elderly are addicted to Cannabis intake abuse despite its predictable toxicological consequences. In this study we studied the toxic effects of oral administration of methanol extract of Cannabis sativa seeds using total of forty male Wistar rats. Animals randomized were into five groups (n=8 rats) of approximately equal weight. Group-1 received 100 mg/kg of the of the extract, group-2 received 200 mg/kg of the extract, group-3 received 300 mg/kg dosage of the extract, group-4 received 2 ml of virgin olive oil (vehicle control) and group-5 received distilled water (normal control) for 14 days. The rationale behind the vehicle control group is to show that the vehicle did not have any extra cytological/histological effect. The proximate properties show: Moisture content (6.69±0.14%), protein content (19.10±0.01%), ash content (11.84±0.02%), fat (19.33±0.00%), crude fiber (18.87±0.00%), carbohydrate (43.04%), dry matter (80.91±0.14%) and oil absorption capacity (1.87±0.00%). The anti-nutrients were as follows: alkaloids (3.15±0.01 mg/100 g), flavonoids (2.82±0.01 mg/100 g), saponins (6.10±0.01%), tannins (2.14±0.01 mg/100 g), cyanogenic glycosides (0.10±0.01 mg/100 g) and phenols (0.27±0.01 mg/100 g). The mineral content of the sample were potassium (13.73±0.01 mg/100 g), phosphorus (10.00±0.02 mg/100 g), sodium (17.53±0.01 mg/100 g), calcium (14.65±0.01 mg/100 g), magnesium (09.67±0.01 mg/100 g), zinc (2.00±0.01 mg/100 g) and Iron (1.00±0.01 mg/100 g). Result for AST was significantly (p<0.05) higher in group-2 (57.00±13.00 IU/L) and group-3 (59.33±10.53 IU/L), when compared with the normal control group-5 (31.33±1.53 IU/L). Significantly (p<0.05) higher serum ALT was observed in treatment group-2 (50.00±12.52 IU/L) and group-3 (56.33±10.21 IU/L). The results for kidney function, shows significantly (p<0.05) higher serum urea concentration in group-3 (13.75±2.41 mg/dl) when compared with the normal control group (8.75±1.60 mg/dl). Serum creatinine concentration was significantly (p<0.05) higher in group-2 (2.25±1.18 mg/kg) and group-3 (2.38±1.57 mg/kg) when compared with the normal control group (1.09±0.13 mg/kg). Significantly (p<0.05) higher SOD values was obtained in group-3 (72.64±5.90 mg/kg) when compared with normal control group (19.62±4.26 mg/kg). There was no significant (p<0.05) difference in total serum protein concentration in all groups when compared with the normal control group. The histological result shows that the oral administration of Cannabis sativa induced pronounced inflammation of the hepatic and renal tissue in group-3 when compared with the normal control group. In conclusion, the study showed that oraladministration of Cannabis sativa caused dose dependent hepatorenal toxicity.